Simple and reliable second order derivative spectrophotometric method was developed and validated for the simultaneous estimation of beclomethasone dipropionate and ketoconazole in combined dosage form. The quantitative determination of the drugs was carried out using the second derivative values measured at 287 nm and 265 nm for beclomethasone dipropionate and ketoconazole respectively. The solutions of standard and the sample were prepared in methanol. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of 5-35 μg/ml for both beclomethasone dipropionate and ketoconazole. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method. Developed second derivative spectrophotometric method was simple, accurate, precise, specific, sensitive and reproducible which can be directly and easily applied to pharmaceutical dosage forms.
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